Preparation chemical probes; Equipment and technology; Preparation and maintinance of biological materials; Use of chemical probes; Quantitation of neuroendocrine substances; Localization of neuroendocrine substances.

Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Structural and functional analysis of oxidative phosphorylation complexes * Import of proteins and RNA into mitochondria * Ion and metabolite transport systems in mitochondria * Biophysical methods for mitochondrial function analysis * Mitochondrial inheritance and turnover.

Methods included in this volume apply to the expression and characterization of retroviral proteases and their inhibitor/substrate design.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Phage display libraries* Repression fusion proteins* Polysome libraries* Peptide libraries* Nucleic acid libraries* Other small molecule libraries.

General Description of the Series: Neurotransmitter Transporters focuses on biochemical, electrophysiological, pharmacological, molecular, and cell biological approaches used to study neurotransmitter transport systems. The articles provide detailed descriptions of procedures that should enable the reader to understand how they are accomplished and to repeat or adapt them for their own experimental needs. This book is the first to focus on methods that have been the basis for the rapid development of this area. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * The transport of CNS neurotransmitter transporters * Electrophysiological, biochemical, molecular, cellular biological, pharmacological, neurochemical, and structural approaches * Both plasma and vesicular carriers

This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.