Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

MATLAB® Primer for Speech-Language Pathology and Audiology provides training and access to MATLAB®, the computational language developed by MathWorks®. While there are MATLAB® textbooks and manuals written for the field of engineering, there are no textbooks targeting allied heath disciplines, particularly speech-language pathology and audiology. Research and practice in this field can greatly benefit from quantification and automation in data management, a domain that is increasingly labor-intensive. The text anticipates and promotes increased reliance on quantification and automation in the fields of speech-language pathology and audiology. This book is intended for students, practitioners, and researchers in speech-language pathology and audiology who wish to increase their productivity by incorporating and automating common research procedures and data-analysis calculations, or who wish to develop new tools and methods for their own paradigms and data processing. It assumes no prior knowledge of programming, but requires the reader to have a grasp of basic computer skills, such as managing folders, moving files, and navigating file paths and folder structures. Content and style are chosen so as to lower the threshold for an audience who has limited training in computer science. Concepts are presented in a personalized writing style (almost a dialogue with the reader), along with a didactic format similar to programmed instruction, using applications and work assignments that are concrete and manageable. Key features include: * A comprehensive introduction for the user in an effort to limit background knowledge needed to understand the content * Several mathematical review appendices * Exercises for the student to apply skills learned in laboratory and clinical applications Disclaimer: Please note that ancillary content (such documents, audio, and video) may not be included as published in the original print version of this book.

This updated and expanded tutorial guide to molecular diagnostic techniques takes advantage of many new molecular technologies to include both improved traditional methods and totally new methods, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that complementary to molecular diagnostic technologies and discuss genetic counseling and the ethical and social issues involved with nucleic acid testing.

Molecular Detection of Animal Viral Pathogens presents expert summaries on state-of-the-art diagnostic approaches for major animal viral pathogens, with a particular emphasis on identification and differentiation at the molecular level. Written by specialists in related research areas, each chapter provides a concise overview of an individual virus

Healthy seeds and propagules are the basic requirement for producing good grains, fruits and vegetables needed for human survival and perpetuation. Dispersal of microbial plant pathogens via seeds and propagules has assumed more importance than other modes of dispersal, as infected seeds and propagules have the potential to become the primary sources of carrying pathogen inoculum for subsequent crops. Several diseases transmitted through seeds and propagules have been shown to have the potential to damage economies as a result of huge quantitative and qualitative losses in numerous crops. Hence, it is essential to rapidly detect, identify and differentiate the microbial plant pathogens present in seeds and propagules precisely and reliably, using sensitive techniques. Microbial Plant Pathogens: Detection and Management in Seeds and Propagules provides a comprehensive resource on seed-borne and propagule-borne pathogens. Information on the biology of microbial pathogens, including genetic diversity, infection process and survival mechanisms of pathogens and epidemiology of diseases caused by them, are discussed critically and in detail to highlight weak links in the life cycles of the pathogens. Development of effective disease management systems, based on the principles of exclusion and eradication of pathogens and immunization of crop plants to enhance the levels of resistance of cultivars to diseases, has been effective to keep the pathogens at bay. The need for production of disease-free seeds/propagules has been emphasized to prevent the carryover of the inoculum to the next crop or introduction of the pathogens to other locations. Effectiveness of adopting simple cultural practices and development of cultivars resistant to diseases through traditional breeding methods or biotechnological approach have resulted in reducing the pathogen inoculum and disease incidence. Although application of different chemicals may reduce the disease incidence effectively, biological management of crop diseases, employing potential biological control agents have to be preferred to preserve the agroecosystems. Greater efforts have to be made to integrate compatible strategies to enhance the effectiveness of diseases management systems. Protocols appended at the end of relevant chapters form a unique feature of this book to enable the researchers to fine-tune their projects. This 2 volume set provides comprehensive and updated information about the economically-important groups of microbial plant pathogens carried by seed and propagules. Graduate students, researchers and teachers of plant pathology, plant protection, microbiology, plant breeding and genetics, agriculture and horticulture, as well as certification and quarantine personnel will find the information presented in this book useful.

This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.