Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

A broad, concise, and no-nonsense guide to contemporary electrophysiological techniques, covering intracellular and extracellular recording through recording of population activity, neuropharmacology, dye imaging, voltammetry, and optogenetics.

This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions. The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches.

Electrophysiological Methods in Biological Research, Third Revised Edition describes the principles and applications of significant electrophysiological methods as regards to transistorisation of electrophysiological apparatus and to the mathematical analysis of electrophysiological data. The book explains the aspects of physics and electronics that are important in electrophysiology, such as the basic principles of semiconductor function, electronic simulators, electrodes, and the processing of electrophysiological data. The text also cites several examples that measure the resulting membrane potential if one electrode is inside the cell while the other is in contact with the cell's surface. Other experiments show the electrophysiological techniques and the fundamentals of electrical activity in the peripheral excitable structures, and its association with physiological functions. In considering the problems of nerve and muscle physiology, the investigator should know the technique of recording the electrical signs of a nerve impulse. These signs, or action potentials, indicate the presence of a nerve impulse. The text also discusses the effects of barbiturates or ether anesthesia in EEG activity, as well as its dissociation after physostigmine and atropine have been administered. The book can prove useful for pharmacologists, microchemists, cellular biologists, and research workers and technologists dealing with neural mechanisms.

The new edition of Electrophysiological Disorders of the Heart helps you diagnose and treat a full range of heart rhythm disorders using today’s latest technologies and therapies. It provides practical, hands-on coverage of hot topics such as pediatric EP, imaging, echocardiography-guided EP procedures, regenerative therapies, cardiac pacing, and more. Now available in a new full-color format, the title also includes easy online access at www.expertconsult.com. Discover new ways to treat and manage the full range of heart rhythm disorders with content focused on common clinical features, diagnosis, and management. Review expert management strategies to help you handle complex patient problems. Stay current with the latest molecular and technical advances as well as new treatment options implemented over the last few years. Use the latest technologies and devices to accurately diagnose and manage heart rhythm disorders. Consult new and expanded coverage of regenerative therapies, echo-guided procedures, cardiac pacing, and CRT, as well as a new section on pediatric electrophysiology and imaging. Enjoy improved visual guidance with many new full-color images. Log on to www.expertconsult.com to easily search the complete contents online and access a downloadable image library.

Introduction to Electrophysiological Methods and Instrumentation, Second Edition covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells and the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments, including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters collected by optical means. This book provides the electrophysiologist with the tools needed to understand his or her equipment and how to acquire and analyze low-voltage biological signals. - Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes - Discusses the particulars of recording from brain tissue slices, oocytes and planar bilayers - Describes optical methods pertinent to electrophysiological practice - Presents the fundamentals of signal processing of analogue signals, spike trains and single channel recordings, along with procedures for signal recording and processing - Includes appendices on electrical safety and foundations of useful mathematical tools

Ion channels are intimately involved in the everyday physiological functions that enable us to live a full and varied life. When disease strikes, malfunction of ion channels or their dependent is often involved, either as the cause or the effect of the illness. Thus, billions of dollars have been, and still are being, invested in research to understand the physiological and pathophysiological functions of ion channels in an attempt to develop novel therapeutic treatments for a wide range of diseases. This book provides a comprehensive overview of ion channel structure and function. It comprises two major parts. Part one is an introductory overview of the ion channel superfamily and the generic aspects of ion channel function. This part also reviews the methodologies by which ion channel function can be studied from the perspective of performing detailed biophysical characterization through to the deployment of high throughput approaches for identifying novel ion channel ligands. Part two of the book provides an in-depth review of the individual ion channel subfamilies and, as such, is subdivided into four broad sections: Voltage-Gated Ion Channels, Extracellular Ligand-Gated Ion Channels, Intracellular Ligand-Gated Ion Channels, and Polymodal-Gated Ion Channels, with each chapter focused on specific family members. These chapters have been written by world leading experts and provide a detailed overview of the structure, biophysics, localization, pharmacology, physiology, and disease relevance of each particular ion channel subfamily. Reviewing both the basic principles of ion channel function and providing a detailed up-to-date review of the phsyiological and pharmacological aspects of individual ion channel sub-families, this book constitutes both an excellent introduction to the field for non-specialists, as well as a highly valuable reference text for experienced researchers already working in the ion channel area.

Basic Neurochemistry: Molecular, Cellular and Medical Aspects, a comprehensive text on neurochemistry, is now updated and revised in its Seventh Edition. This well-established text has been recognized worldwide as a resource for postgraduate trainees and teachers in neurology, psychiatry, and basic neuroscience, as well as for graduate and postgraduate students and instructors in the neurosciences. It is an excellent source of information on basic biochemical processes in brain function and disease for qualifying examinations and continuing medical education. - Completely updated with 60% new authors and material, and entirely new chapters - Over 400 fully revised figures in splendid color

Scientists working or planning to work in the field of cardiovascular research will welcome Practical Methods in Cardiovascular Research as the reference book they have long been waiting for. Not only general aspects of cardiovascular research are well presented, but also detailed descriptions of methods and protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Practical Methods in Cardiovascular Research is therefore a long awaited "must have" for anybody with an interest in cardiovascular research.

Neuroprosthetics is an area of intense scientific and clinical interest and rapid progress. Since the introduction of the cardiac pacemaker in 1932, we have seen developments that include cochlear prostheses, techniques for bladder and bowel control, deep brain stimulation, and restoration of mobility and respiration to paralyzed individuals.The chapters in this book have been contributed by authors who are recognized internationally in their fields. The result is a comprehensive and up-to-date review that will be invaluable to graduate students, clinicians and researchers in neuroprosthetics. It is broadly divided into three sections: Section 1 provides a core of knowledge that forms a foundation for the rest of the book, and covers the basics of neuroanatomy and neurophysiology, biomaterials and biocompatibility, stimulation and recording techniques; Section 2 describes current clinical applications of neuroprosthetics; Section 3 looks at future developments in the field.