Bone Marrow Processing and Purging: A Practical Guide provides an up-to-date practical guide to the major ex vivo procedures associated with bone marrow transplantation. Previously, this information was communicated primarily by word of mouth; now experts in the field present detailed descriptions and evaluations of methods for marrow harvesting, evaluation (including tumor infiltration, flow cytometric analysis, and colony assays), comparative methods for automated nucleated cell separation and enumeration, tumor cell purging, T cell depletion, stem cell selection, gene transfer, and cytopreservation. Special sections address quality control and FDA regulations. The book provides a unique information source intended for clinicians, researchers, technical staff, transplant nurses, and medical students involved in this rapidly expanding area of medicine.

This volume assesses research findings as well as the clinical aspects now in use for selectively separating subpopulations of cells from bone marrow. It examines a full range of purging processes including transplantation programs, cryopreservation, cell isolation and cell growth, specificity testing and quality control, as well as the development of alternative sources of stem cells, including peripheral blood and cadaveric marrow. It also presents the conditions necessary to optimize graft toxicity and bone marrow transportation and addresses newer techniques of gene probing/processing as well as mechanisms of genetic alteration including electroporation.

Surveys the latest research discoveries and current clinical procedures for selectively separating and analyzing subpopulations of cells from bone marrow. Over 400 internationally renowned experts offer contributions on such topics as approaches for using DNA sense and antisense probes, combinations of elutriators and immunomagnetics, expanded cytokines applications, new techniques for the removal of tumor cells from autologous bone marrow and T cells from allogeneic grafts.

Examines emerging technology in this field, from both the research and clinical arenas. The book describes approaches for utilizing DNA sense and antisense probes, combinations of elutriators and immunomagnetics as well as refinements in the use of mechanical separation. Contributions from more than 400 investigators address topics such as pharmacological purging, marrow processing, clinical studies and tumor cell detection.

At the time of the first edition of Principles of Cancer Biotherapy, this book represented the first comprehensive textbook on biological therapy. Whereas in 1991, when the second edition was published, there was still some doubt on the part of many oncologists and cancer researchers as to the therapeutic value of these new approaches, it is now generally agreed that biopharmaceuticals are producing major opportunities for new cancer therapies. Cancer biotherapy has truly matured into the fourth modality of cancer treatment. The third edition is now needed as a result of the tremendous progress that has been made in recent years using biologicals in cancer treatment. The book summarizes an evolving science and a rapidly changing medical practice. As we near the millennium, it now becomes possible to envision a much more diversified system of cancer research and treatment that will afford greater opportunities for patients. Some forms of cancer biotherapy use the strategy of tumour stabilization and control through continued biological therapy, akin to the use of insulin in the treatment of diabetes. This textbook illustrates new methods of thinking and new strategies for control of cancer. It is always difficult to move from past dogma to future opportunity, but this third edition of Principles of Cancer Biotherapy illustrates why it is so important to the patients, for researchers and clinicians to explore and apply these new opportunities in cancer biotherapy.

At the time of the first edition of Principles of Cancer Biotherapy in 1987, this book represented the first comprehensive textbook on biological therapy. In 1991, when the second edition was published, there was still some doubt on the part of many oncologists and cancer researchers as to the therapeutic value of these new approaches. By 2003 and the fourth edition, it was generally agreed that biopharmaceuticals were producing major opportunities for new cancer therapies. Cancer biotherapy has now truly matured into the fourth modality of cancer treatment. This fifth revised edition describes the tremendous progress that has been made in recent years using biologicals in cancer treatment. This book summarizes an evolving science and a rapidly changing medical practice in biotherapy. In this new millennium, it is now possible to envision a much more diversified system of cancer research and treatment that will afford greater opportunities for a patient’s personalized cancer treatment. This was first envisioned in the 1987 initial edition of this textbook and is now a “new” and popular approach to cancer treatment. Some forms of cancer biotherapy use the strategy of tumor stabilization and control through continued biological therapy, akin to the use of insulin in the treatment of diabetes. This textbook illustrates new methods of thinking and new strategies for control of cancer. It is always difficult to move from past dogma to future opportunity, but this fifth edition of Principles of Cancer Biotherapy illustrates why it is so important to the patients for researchers and clinicians to explore and quickly apply these new opportunities in cancer biotherapy.

"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Handbook of Transfusion Medicine is unique in that it provides a comprehensive and practical description of all blood products and blood cell types currently used in transfusions, their appropriate applications, pathophysiology of conditions managed by transfusion, and pathophysiology of adverse reactions. Each chapter follows a standard format including numerous tables and algorithms, with summary elements highlighted throughout by a second-color for quick reference.Sections Include:* Blood collection and testing* Blood component description* Preparation and usage* Red blood cell antigens and antibodies* Specialized component processing* Specialized transfusion situations* Transfusion-transmitted diseases* Transfusion reactions* Infectious complications of transfusion* Therapeutic apheresis and quality* Acute bleeding and massive transfusion* Transfusion of the patient with a coagulopathy* Transfusion of obstetrics, pediatric, immunocompromised, and platelet refractory patients* Up-to-date references to all aspects of transfusion medicine