This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed. From the contents: * Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond * New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors * Monooxygenase-Catalyzed Redox Cascade Biotransformations * Biocatalytic Redox Cascades Involving w-Transaminases * Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases * Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates * Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids * Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution * Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode * Perspectives on Multienzyme Process Technology * Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions * Mining Genomes for Nitrilases * Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion * Enzymatic Stereoselective Synthesis of Beta-Amino Acids * New Applications of Transketolase: Cascade Reactions for Assay Development * Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs * Enzymatic Generation of Sialoconjugate Diversity * Methyltransferases in Biocatalysis * Chemoenzymatic Multistep One-Pot Processes

This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed. From the contents: * Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond * New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors * Monooxygenase-Catalyzed Redox Cascade Biotransformations * Biocatalytic Redox Cascades Involving w-Transaminases * Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases * Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates * Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids * Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution * Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode * Perspectives on Multienzyme Process Technology * Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions * Mining Genomes for Nitrilases * Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion * Enzymatic Stereoselective Synthesis of Beta-Amino Acids * New Applications of Transketolase: Cascade Reactions for Assay Development * Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs * Enzymatic Generation of Sialoconjugate Diversity * Methyltransferases in Biocatalysis * Chemoenzymatic Multistep One-Pot Processes

This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Chemo-Enzymatic Cascade Reactions A groundbreaking book focusing on chemo-enzymatic cascade transformations Chemo-Enzymatic Cascade Reactions offers a unique book that explores biocatalytic-chemical cascade reactions and their applications in the synthesis of valuable chemicals. Written by a noted expert on the topic, this comprehensive resource includes information on the advantages and disadvantages of traditional chemical and biocatalytic reactions and reviews the three modes of chemo-enzymatic transformations: separate-pot-two-step, one-pot-two-step, and one-pot-one-step. The author examines the most current developments of chemo-enzymatic transformations organized by the three modes and types of enzymes and considers retro-synthesis based on both chemical and biocatalytic transformations and the synthetic applications. This groundbreaking book is the first resource to present in one volume the state-of-art advances of the technology and explore the opportunities and challenges of this burgeoning field. The book also considers the future of cascade reactions and the myriad benefits including higher atom economy and production efficiency, and less resource consumption and waste generation. This important book: Offers the first book dedicated exclusively to chemo-enzymatic cascade transformations Explains the importance and the opportunities and challenges of chemo-enzymatic synthetic technology Includes information on the three modes of chemo-enzymatic transformation Reviews the most recent advances in the field Written for organic chemists, chemists in industry, biochemist, catalytic chemists, Chemo-Enzymatic Cascade Reactions offers an understanding to the importance, current advances, the opportunities and challenges of chemo-enzymatic synthetic technology.

Designer Microbial Cell Factories: Metabolic Engineering and Applications, the latest release in the Current Developments in Biotechnology and Bioengineering series, provides a detailed overview of the biotechnological approaches and strategies used to generate engineered microbes and to facilitate the acceleration, modulation and diversion of metabolic pathways to get desired output such as production of value-added compound or biodegradation of xenobiotic pollutant. The book also highlights applied aspects of designer microbes in fields as diverse as agriculture, pharmaceuticals and bioremediation. Designer microbes generated through reprogramming the microbial cell factories (MCFs) provide an edge over their natural counterparts in terms of increased molecular diversity and selective chemistry. These bugs are becoming instrumental in several areas, including agriculture, environment and human health. Engineering microbes through directed evolution not only gives freedom from evolutionary constrains but also allow introduction of regulated and foreseeable functions into MCFs. Dedicated to the designing of microbes, covering state-of-the-art technological advancements in the field Includes applications of metabolic engineering in the field of agriculture, bioremediation, value-added products, therapeutics, and more Contains chapters dedicated to innovative approaches surrounding engineered microbial consortia Provides comprehensive details and helps users understand concepts

This book provides a comprehensive overview of the recent developments achieved in the field of chemo/enzymatic cascades with topics spanning from design (in vitro and in vivo) to kinetic- and process modelling as well as process control. Opportunities and challenges of building multi-step chemo/enzymatic reactions are discussed, whereby the latter are critically assessed in each chapter and methods to ease the implementation are explored. Both, multi-enzymatic cascades and chemo-enzymatic cascades are presented with the motivation of combining the strengths of these two worlds (e.g. selectivity, activity and robustness) not neglecting the obstacles and challenges of such endeavour. Furthermore, the use of non-conventional media for catalytic cascade reactions, recent achievements and potential for future developments in a technical environment are addressed.

Advances in Enzyme Catalysis and Technologies intends to provide the basic structural and functional descriptions, and classification of enzymes. The scientific information related to the recombinant enzyme modifications, discovery of novel enzymes and development of synthetic enzymes are also presented. The translational aspects of enzyme catalysis and bioprocess technologies are illustrated, by emphasizing the current requirements and future perspectives of industrial biotechnology. Several case studies are included on enzymes for biofuels application, micro algal biorefineries, high-value bioactive molecules production and enzymes for environmental processes, such as enzymatic bioprocessing for functional food development, biocatalytic technologies for the production of functional sweetener, etc. - Provides a conceptual understanding of enzyme catalysis, enzyme engineering, discovery of novel enzymes, and technology perspectives - Includes comprehensive information about the inventions and advancement in enzyme system development for biomass processing and functional food developmental aspects - Gives an updated reference for education and understanding of enzyme technology

Green Biocatalysis presents an exciting green technology that uses mild and safe processes with high regioselectivity and enantioselectivity. Bioprocesses are carried out under ambient temperature and atmospheric pressure in aqueous conditions that do not require any protection and deprotection steps to shorten the synthetic process, offering waste prevention and using renewable resources. Drawing on the knowledge of over 70 internationally renowned experts in the field of biotechnology, Green Biocatalysis discusses a variety of case studies with emphases on process R&D and scale-up of enzymatic processes to catalyze different types of reactions. Random and directed evolution under process conditions to generate novel highly stable and active enzymes is described at length. This book features: A comprehensive review of green bioprocesses and application of enzymes in preparation of key compounds for pharmaceutical, fine chemical, agrochemical, cosmetic, flavor, and fragrance industries using diverse enzymatic reactions Discussion of the development of efficient and stable novel biocatalysts under process conditions by random and directed evolution and their applications for the development of environmentally friendly, efficient, economical, and sustainable green processes to get desired products in high yields and enantiopurity The most recent technological advances in enzymatic and microbial transformations and cuttingedge topics such as directed evolution by gene shuffling and enzyme engineering to improve biocatalysts With over 3000 references and 800 figures, tables, equations, and drawings, Green Biocatalysis is an excellent resource for biochemists, organic chemists, medicinal chemists, chemical engineers, microbiologists, pharmaceutical chemists, and undergraduate and graduate students in the aforementioned disciplines.

Implementing biocatalytic strategies in an industrial setting at a commercial scale is a challenging task, necessitating a balance between industrial need against economic viability. With invited contributions from small and large-scale chemical and pharmaceutical companies, this book bridges the gap between academia and industry. Contributors discuss current processes, types of biocatalysts and improvements, industrial motivation and key aspects to economically succeed. With its focus on industry related issues, this book will be a useful tool for future research by both practitioners and academics.

This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.