The first in-depth, critical look at figure skating.

It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.

We proudly present the proceedings of 4th International Conference on Economics, Business and Economic Education Science 2021 (ICE-BEES 2021). It has focus on the innovations in economics, business, education, environment, and sustainable development. The issue of economics and sustainable development is important today. Especially in the time of Covid-19. Not only globally, but also Indonesia nationally to the local level. There are several important issues relating to this, both institutionally and the relationships between individuals and groups in supporting the agenda of sustainable development. More than 200 manuscripts were presented at this conference with 101 of them selected to be published in proceedings. We hope by this conference, discussions on the importance of sustainable development will increasingly become an important concern together. Brings better response from the government and social relations for development.

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.

Arguing that Antarctica is the most mediated place on earth and thus an ideal location for testing the limits of bio-political management of population and place, this book remaps national and postcolonial methods and offers a new look on a 'forgotten' continent now the focus of ecological concern.

Cross-cultural management is an important facet of the globalized sport industry. Sport managers must be skilled at working with individuals from diverse cultures and aware of the key issues affecting sport on a global level. This book brings together cutting-edge research from leading sport scholars from around the world, to illuminate some of those important issues and to demonstrate what cross-cultural management looks like in a sporting context. Presenting case studies from countries as diverse as the US, Brazil, Poland and Venezuela, and across a range of sports from football to basketball, the book presents new empirical material derived from a range of inquiry protocols, including both qualitative and quantitative methods. It offers critical analyses of cross-cultural and managerial issues in key areas such as group cohesiveness, group communications, and misperception and misinterpretation. Making an important contribution to our understanding of both theory and practice in sport management, this book is fascinating reading for any student, researcher or practitioner with an interest in global and international sport.

[Kit] provides session plans, fact sheets, and other resources to help tailor HIV/AIDS training in pre-service training and in-service training to the needs of various groups of trainees and Volunteers.

The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.