This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half. - Shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy - Contains 10 new chapters addressing developments over the last several years - Covers basic principles, applications, and equipment - Spcialized video microscopy techniques are covered

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. - Expands coverage to include discussion of confocal microscopy not found in the previous edition - Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. - Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope - Clearly develops all ideas from historical and logical foundations - Laboratory exercises included to assist the reader with practical applications - Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Rehabilitation of heritage monuments provides sustainable development and cultural significance to a region. The most sensitive aspect of the refurbishment of existing buildings lies in the renovation and recovery of structural integrity and public safety. The Handbook of Research on Seismic Assessment and Rehabilitation of Historic Structures evaluates developing contributions in the field of earthquake engineering with regards to the analysis and treatment of structural damage inflicted by seismic activity. This book is a vital reference source for professionals, researchers, students, and engineers active in the field of earthquake engineering who are interested in the emergent developments and research available in the preservation and rehabilitation of heritage buildings following seismic activity.

Making a clear distinction is made between nano- and micro-mechanical testing for physical reasons, this monograph describes the basics and applications of the supermicroscopies AFM and SNOM, and of the nanomechanical testing on rough and technical natural surfaces in the submicron range down to a lateral resolution of a few nm. New or improved instrumentation, new physical laws and unforeseen new applications in all branches of natural sciences (around physics, chemistry, mineralogy, materials science, biology and medicine) and nanotechnology are covered as well as the sources for pitfalls and errors. It outlines the handling of natural and technical samples in relation to those of flat standard samples and emphasizes new special features. Pitfalls and sources of errors are clearly demonstrated as well as their efficient remedy when going from molecularly flat to rough surfaces. The academic or industrial scientist learns how to apply the principles for tackling their scientific or manufacturing tasks that include roughness far away from standard samples.

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques.Practical laboratory guide for methods and technologies used with video microscopyComprehensive, easy-to-follow instructionsFebruary 1998, c. 334 pp.

Now in full color with hundreds of new images, Laboratory Procedures for Veterinary Technicians, 6th Edition covers the broad spectrum of laboratory procedures that veterinary technicians need to perform effectively in the practice setting. Comprehensive content presents the fundamentals of microbiology, hematology, urinalysis, immunology, and cytology, along with the laboratory procedures used to perform the most widely used tests such as complete blood count, urinalysis, and immunologic assays. This edition includes newly organized chapters with expanded coverage of essential material to prepare you for real-life laboratory work. "Everything you would expect from an in- house lab is listed here." Reviewed by Fabienne Dethioux on behalf of Vet Nurses Today, March 2015 Comprehensive coverage gives you a solid foundation in the fundamentals of microbiology, hematology, urinalysis, immunology, and cytology, along with the laboratory procedures used to perform related tests. Step-by-step procedure boxes offer quick access to the skills you must perform during your educational program, as well as procedures that are commonly performed by vet techs in private practice. Provides the latest information needed to successfully perform a broad spectrum of laboratory tests, including complete blood count, urinalysis, and immunologic assays. Completely updated content throughout reflects the latest advances in veterinary clinical laboratory procedures for improved patient service and higher practice revenue. A comprehensive glossary of terms at the end of the text offers accurate, concise definitions and phonetic pronunciation guides. NEW! Streamlined chapters are shorter and easier to digest with expanded coverage of essential material to prepare you for real-life laboratory work. NEW! Full-color photos bring concepts to life, sequentially arranged to illustrate step-by-step procedures for all commonly performed diagnostic tests in the clinical laboratory. NEW! Companion Lab Manual (sold separately) includes multiple-choice questions, fill-in-the-blank exercises, photo quizzes, labeling exercises, crossword puzzles, and other activities to help you master and apply key concepts and procedures in clinical situations. NEW! Special emphasis on the significance of abnormal results of key lab tests, zoonoses, and hematology. NEW! Vet Tech Threads provide you with introductions, suggested readings, boxed technician notes, learning objectives, chapter outlines, key terms, and a glossary for easy navigation through chapters and more focused learning.

Microscopy is a dynamic area of science, incorporating both basic classroom microscopes and sophisticated research style instruments that can be driven by light, electrons, or X-rays. The rate of advance in the area over the last 50 years has led to a number of technological advances. In this Very Short Introduction Terence Allen, an established expert on microscope techniques, describes the scientific principles behind the main forms of microscopy, and the exciting new developments in the field. Focusing on the main underlying principles, and introducing the power of what is achievable today using microscopes, Allen demonstrates how microscopy impinges on almost every aspect of our daily lives; from medical diagnosis to quality control in manufacture. Beginning with a brief history of the early stages of microscopy development, Allen then concludes with a comprehensive account of the diverse spectrum of microscopy available today. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.

Handbook of Optical Metrology: Principles and Applications begins by discussing key principles and techniques before exploring practical applications of optical metrology. Designed to provide beginners with an introduction to optical metrology without sacrificing academic rigor, this comprehensive text: Covers fundamentals of light sources, lenses, prisms, and mirrors, as well as optoelectronic sensors, optical devices, and optomechanical elements Addresses interferometry, holography, and speckle methods and applications Explains Moiré metrology and the optical heterodyne measurement method Delves into the specifics of diffraction, scattering, polarization, and near-field optics Considers applications for measuring length and size, displacement, straightness and parallelism, flatness, and three-dimensional shapes This new Second Edition is fully revised to reflect the latest developments. It also includes four new chapters—nearly 100 pages—on optical coherence tomography for industrial applications, interference microscopy for surface structure analysis, noncontact dimensional and profile metrology by video measurement, and optical metrology in manufacturing technology.