This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.

Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infections. The book discusses the fundamentals of Molecular Diagnostics for bacteria, fungi, viruses and a variety of state-of-the-art diagnostics tools and techniques for obtaining qualitative and quantitative results. It includes topics such as Next generation sequencing and application of "OMICS" for early disease diagnosis. Sections cover the entire spectrum of emerging diagnostic tools and techniques, as well as the principles, pros and cons of each method and applications for diagnosis of infectious disease in plants, humans and veterinary. This is a resource for both researchers and students working in clinical microbiology, infectious biology, applied life sciences and scientists working in the clinical diagnostic industry. - Provides a wide range of diagnostics tools and technologies in the field of infectious biology - Presents discussions on emerging technologies for early disease diagnosis and novel diagnostics techniques for detection of COVID, Urea Breath Tests for Detection of Helicobacter pylori, Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis, Molecular imaging, Lab-on-a-chip Technologies, and several others - Analyzes literature reviews, underpinning methodologies, and opportunities and limitations - Includes case studies and examples that highlight applications using the underpinning techniques

**Selected for Doody's Core Titles® 2024 with "Essential Purchase" designation in Veterinary Medicine** Greene's Infectious Diseases of the Dog and Cat, 5th Edition provides a comprehensive, clinically useful reference on the management of infectious diseases caused by viruses, bacteria (including rickettsiae, chlamydiae, mycoplasmas, and spirochetes), fungi, algae, protozoa, parasites, and other atypical agents. Each section guides the reader through diagnostic testing for specific infectious diseases, from specimen collection to laboratory submission to interpretation of results to appropriate treatment measures. Full-color illustrations and hundreds of tables provide convenient access to diagnostic and therapeutic recommendations, along with the appropriate drug dosages for effective treatment and prevention. A fully searchable enhanced eBook version is included with print purchase, allowing access to all of the text and figures on a variety of digital devices. - More than 150 internationally recognized experts contribute chapters on topics in their field of specialty. - Clear and logical organization of chapters provides a solid basis for an approach to diseases caused by specific pathogens, with the first part of the book including sections on diagnostic approaches, treatments (including recommended antimicrobial drug doses), and prevention. - Specific pathogens are addressed in the second part of the book, using a structured approach that includes etiology/epidemiology (relevance to wildlife animal hosts, role of the environment), clinical and laboratory findings, treatment, prevention, and public health implications. - Case examples illustrate principles and highlight how the material can be applied. - More than 800 clinical images, maps, life cycles, and photomicrographs assist with accurate understanding of epidemiology, pathogenesis, diagnosis of disease, and disease prevention. - Visually appealing maps and life-cycle drawings enhance your comprehension and retention of the material. - Convenient drug dosage tables in each chapter provide complete prescribing information; chapters on antimicrobial drugs in the first part of the book summarize pharmacokinetics, indications, contraindications, handling and administration guidelines; and dosage recommendations are made for antivirals, antibacterials, antifungals, antiprotozoals, and antiparasitic drugs. The book emphasizes approaches to optimize antimicrobial stewardship. - Clinical Problems section helps you understand what infectious diseases should be considered in animals seen with clinical signs relating to different organ systems. - Suggested readings and references are listed in each chapter, facilitating further research and study. - Fully searchable enhanced eBook version is included with print purchase, allowing access to all of the text and figures on a variety of digital devices.

New edition presenting latest developments in ophthalmic diagnostic procedures. Fully revised and many new chapters. Previous edition published in 2009.

This book offers a timely and comprehensive review of essential research on Peste des Petits Ruminants Virus (PPRV), ranging from its historical distribution, molecular epidemiology, genome structure, viral proteins, immunity, viral pathogenesis, clinical and molecular diagnosis to advances in vaccine developments and future challenges. PPRV, a Rinderpest-like virus, is the causative agent of one of the most rapidly emerging viral diseases among domestic small ruminants, and the host spectrum has now been expanded to wild small ruminants and camels. With the global eradication of the first livestock disease, Rinderpest, attention is now turning to repeating the procedure for PPR. Each of the book’s 13 chapters is dedicated to a specific topic, providing up-to-date literature and discussions by renowned scientists who have made seminal contributions in their respective fields of expertise. Special emphasis has been placed on the analysis of different global efforts to eradicate PPR. This book offers a valuable reference source for virologists, field veterinarians, infection and molecular biologists, immunologists, scientists in related fields and veterinary school libraries.

This beautifully illustrated, comprehensive reference provides concise information on the materials and methods of bacteriology, mycology, and virology. The book covers the collection, isolation, and culture of diagnostic specimens, with detailed notes on the biochemical, serological and other tests currently used to identify and distinguish between microbial pathogens. The new edition sets out to provide the most up-to-date account of all the clinically and economically important pathogens, including Bovine Spongiform Encephalomyeltis, Creutzfeldt-Jakob Disease, E-coli, and Salmonella. The clear, accessible format, together with the complete revision of the content, makes this a valuable resource. - High quality full colour photography - Essential for accurate diagnosis - Fully revised pathogenicity sections taking into account the major discoveries/incidences of the last 3-5 years - Reclassification of viruses, including changes to nomenclature - Appendices of Infectious Diseases - Fast access to vital information - Unique and practical inclusion of virology, bacteriology and mycology in one text - Greatly expanded chapter on viruses - More on PRIONS (including BSE) - Reclassification of viruses - many changes to nomenclature - Fully revised pathogenicity sections - Revised/complete coverage of E coli 0157 - Revised Systems section - Complete update of Infectious Diseases coverage in the appendices

The definitive bible for the field of biomedical engineering, this collection of volumes is a major reference for all practicing biomedical engineers and students. Now in its fourth edition, this work presents a substantial revision, with all sections updated to offer the latest research findings. New sections address drugs and devices, personalized medicine, and stem cell engineering. Also included is a historical overview as well as a special section on medical ethics. This set provides complete coverage of biomedical engineering fundamentals, medical devices and systems, computer applications in medicine, and molecular engineering.

This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.

Tissue engineering research continues to captivate the interest of researchers and the general public alike. Popular media outlets like The New York Times, Time, and Wired continue to engage a wide audience and foster excitement for the field as regenerative medicine inches toward becoming a clinical reality. Putting the numerous advances in the fi