This work is written to provide a qualitative introduction, appropriate for a general science audience, to the application of pragmagnetic resonance to the determination of biomolecular dynamics. The work is also intended as a reference resource for those pursuing or contemplating research in the hydrodynamics. The work is also intended as a reference resource for those pursuing or contemplating research in the hydrodynamic characterization of components of Biosystems. Thus, the Introduction, Theory, and Methodology sections involve presentations at two levels a pictorial and intuitive presentation for the generalist and a quantitative presentation for the specialist. The sections on applications provide a critical discussion of both pure and applied research applications which yields insights into both the capabilities and limitations of the methodology. The applications sections are also of interest from the standpoint of the detailed characterization of certain Biosystems, such as erythrocytes, which have evolved from EPR measurements.

This work is written to provide a qualitative introduction, appropriate for a general science audience, to the application of pragmagnetic resonance to the determination of biomolecular dynamics. The work is also intended as a reference resource for those pursuing or contemplating research in the hydrodynamics. The work is also intended as a reference resource for those pursuing or contemplating research in the hydrodynamic characterization of components of Biosystems. Thus, the Introduction, Theory, and Methodology sections involve presentations at two levels a pictorial and intuitive presentation for the generalist and a quantitative presentation for the specialist. The sections on applications provide a critical discussion of both pure and applied research applications which yields insights into both the capabilities and limitations of the methodology. The applications sections are also of interest from the standpoint of the detailed characterization of certain Biosystems, such as erythrocytes, which have evolved from EPR measurements.

Advanced EPR: Applications in Biology and Biochemistry provides an up-to-date survey of existing EPR techniques and their applications in biology and biochemistry, and also provides a wealth of ideas for future developments in instrumentation and theory. The material is broadly organized into four parts. In the first part (chapters 1 to 6) pulsed EPR is discussed in detail. The second part (chapters 7 to 12) provides detailed discussions of a number of novel and experimental methods. The third part comprises seven chapters on double-resonance techniques, five on ENDOR and two on optically- and reaction yield-detected resonance. The final part is devoted to a thorough discussion of a number of new developments in the application of EPR to various biological and biochemical problems. Advanced EPR will interest biophysicists, physical biochemists, EPR spectroscopists and others who will value the extensive treatment of pulsed EPR techniques, the discussion of new developments in EPR instrumentation, and the integration of theory and experimental details as applied to problems in biology and biochemistry.

Biomedical EPR – Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling Motion in Biological Membranes Applications to Structure Determination in Proteins Discussion of Trends in EPR Technology and Prognosis for the Future

Electron magnetic resonance spectroscopy is undergoing something akin to a renaissance that is attributable to advances in microwave circuitry and signal processing software. EPR: Instrumental Methods is a textbook that brings the reader up to date on these advances and their role in providing better experimental techniques for biological magnetic resonance. Chapters in this book guide the reader from basic principles of spectrometer design through the advanced methods that are providing new vistas in disciplines such as oximetry, imaging, and structural biology. Key Features: Spectrometer design, particularly at low frequencies (below X-band), Design of spectrometer components unique to ENDOR and ESEEM, Optimization of EMR spectrometer sensitivity spanning many octaves, Algorithmic approach to spectral parameterization, Application of Fourier Methods to polymer conformation, oximetry, and imaging.

In Vivo EPR (ESR) is a textbook on this relatively new subject in biomedical electron spin resonance. While a few chapters have appeared in special topics volumes in this series, this book covers the principles and theory, instrumentation as well as the latest applications at the time of its writing. The authors are world-renowned experts and pioneers in their fields. This book is divided into two major sections dealing with theory and instrumentation, and aspects of biochemistry, in vitro and in vivo applications. A significant amount of detail is devoted to clinical applications and the problems and pitfalls encountered in in vivo spectroscopy and imaging. Key Features: -History of In Vivo EPR, -Principles of Imaging-Theory and Instrumentation, -Time-domain Radio Frequency EPR Imaging, -The Measurement of Oxygen In Vivo Using In Vivo EPR Techniques, -Potential Medical (Clinical) Applications of EPR, -Combining NMR and EPR/ESR for In Vivo Experiments.

This handbook is a guide for workers in analytical chemistry who need a starting place for information about a specific instrumental technique. It gives a basic introduction to the techniques and provides leading references on the theory and methodology for an instrumental technique. This edition thoroughly expands and updates the chapters to include concepts, applications, and key references from recent literature. It also contains a new chapter on process analytical technology.

We present this special topics volume on an area which has not received thorough coverage for over 12 years. Spin Labeling: Theory and Applications represents a complete update on new theoretical aspects and applications of the spin-label method. In the "line-shape theory" sections, we are especially pleased to include an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations. Barney Bales discusses inhomogeneous broadening phenomena in detail. Several developments in techniques and interpretation in saturation transfer spectroscopy have appeared since the publica tion of Spin Labeling II: Theory and Applications (L. J. Berliner, ed., Academic Press, 1979). We have included an up-to-date chapter on spin-label applications by M. A. Hemminga and P. A. de Jager. By incorporating 15N and deuterium into nitroxide spin labels, several unique advantages are derived in line-shape analysis. Albert Beth and Bruce Robinson have contributed a detailed chapter on the analysis of these labels in the slow-motion regime while Jane Park and Wolfgang Trommer present the advantages for specific biochemical examples in our "applications" section. Derek Marsh's contri bution on spin-label spectral analysis may be regarded as a summary chapter which touches on several of the detailed spectral analysis methods described in the earlier chapters.