They're the hottest men on ice...and they're falling fast. BYLINES & BLUE LINES A one-night stand hot enough to melt the hardest ice… Brody Mitchell is a damn good hockey player with the NHL contract to prove it. He loves the game but hates the hype, the spotlight and the puck bunnies trying to score an athlete husband. He’s been burned before. He’s not looking to repeat that mistake. But one night with an anonymous beautiful brunette and it’s game on. HARD LINES & GOAL LINES The odds are stacked against them… As the backup goalie for the Philadelphia Colonials, Tim Stanton earned his nickname “Tank” by being an immovable force in net. Hockey has always come first in his life but, at thirty-one, he has an expiration date practically stamped on his ass. It’s time to make hard decisions about his future. Retire on his terms or play until his body gives out? And if he stays in net, will he miss his shot to pursue the woman he loves? DEADLINES & RED LINES Looking to score love... Sugar Donahue is determined to live life on her own terms, even if that means working three dead-end jobs to make ends meet. The only bright spot in her day is the moment the hottest player on the Philadelphia Colonials hockey team walks into the diner where she works. Too bad he’s so far out of her league he might as well be on another planet. RJ Mitchell wants Sugar any way he can have her—on a table, up against a wall, whatever he can get. He’s at the top of his game and a fan favorite, but his life feels stuck in neutral. He’s not willing to risk his heart, but he’ll take whatever Sugar’s willing to give. Three full-length, standalone hockey romances sure to melt the hardest ice.

The Larson icehouse had stood abandoned on the south shore of Black Wolf Lake for the past eighty years. During that time, the building had a rather checkered, some might say sordid past. And now, in the spring of 1980, evil had apparently taken up residence. Jessica Wiggins, Stewart Gregory, and Caleb Anders, eighth graders at Immanuel Lutheran School in Longville, Minnesota, were enjoying their last spring break before starting high school in the fall. Jess had a faith statement to prepare for church, Stewart had a project to complete for the science fair, and Caleb . . . well, Caleb was bored. But Caleb was never bored for long. Some strange men had occupied the icehouse. Caleb's curiosity was an itch that needed scratching. Over the next five days, with Jess and Stewart's help, he would determine who these men were and why their mission was so secretive. In the process, he would put himself in grave danger. There are singular moments in our lives when our actions can truly make a difference. In the spring of 1980, the activities at the Larson icehouse would provide one of these moments in Caleb's short life.

This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.