Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

This handbook volume is part of the Reference Series: Micro/Nano Technologies. This individual volume provides a comprehensive, self-contained, and authoritative knowledge in nanomedicine. It covers the theoretical and practical aspects of functionalizations of nanoparticles as nanomedicine, including surface activation, characterization, and microemulsion systems. As a Springer Major Reference Work it is continuously updated online, allowing newcomers and other readers to keep in touch with the most up-to-date information. The book is particularly recommended to undergraduates, postgraduates, researchers, scientists, and field experts. It will inspire innovations in the highly interdisciplinary field of nanomedicine.

Algae Biotechnology: Integrated Algal Engineering for Bioenergy, Bioremediation, and Biomedical Applications covers key applications of algae for bioenergy and how to integrate the production of biofuels with environmental, nutraceutical and biomedical processes and products. The book emphasizes cost-effective biofuels production through integrated biorefinery, combining continuous processes and various algae as feedstock to produce biofuel, bioenergy and various high value biochemicals. Novel algal culturing technologies and bioprocess engineering techniques are provided for the optimization of operational approaches for commercial-scale production, as well as to reduce the overall costs. New and existing molecular methods for genetic and metabolic engineering of algae are also presented. Furthermore, methods for the optimization of existing biochemical pathways are explained, and new pathways are introduced, in order to maximize the potential for biofuels production and related nutraceutical and biomedical co-products. This book provides an ideal roadmap for bioenergy researchers and engineers who want to incorporate valuable nutraceutical and biomedical products and environmental practices into the production of biofuels. - Addresses issues faced by the bioenergy sector and how to resolve them through the integration of algal biotechnology and engineering - Provides a guide to the efficient and cost-effective production of bioenergy, while simultaneously mitigating pollution and producing valuable nutraceutical and biomedical biproducts - Covers new and emerging approaches in integrated algal biotechnology - Offers a roadmap to their application in the production of biofuels alongside nutraceutical, biomedical, and environmental processes and products

Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions

This comprehensive handbook provides up-to-date knowledge and practical advice from established authorities in aerosol science. It covers the principles and practices of bioaerosol sampling, descriptions and comparisons of bioaerosol samplers, calibration methods, and assay techniques, with an emphasis on practicalities, such as which sampler to use and where it should be placed. The text also offers critiques concerning handling the samples to provide representative and meaningful assays for their viability, infectivity, and allergenicity. A wide range of microbes-viz., viruses, bacteria, fungi and pollens, and their fragments-are considered from such perspectives. Bioaerosols Handbook is divided into four parts, providing a wide-ranging reference work, as well as a practical guide on how best to sample and assay bioaerosols using current technology.

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

The term microalgae is often used in the algal research community to collectively describe microscopic algae and cyanobacteria. Research of microalgae has expanded enormously, namely because of their significant commercial potential. The thorough knowledge of the physiology of microalgae must precede any commercial exploitation. We have to understand the mechanisms underlying the physiological and biochemical processes in the algal cells. The book Microalgae - From Physiology to Application covers major aspects of microalgae physiology and the possible applications in the sphere of biotechnology. This book gives a comprehensive overview of what is known about microalgae growth and production, secondary metabolites, and development of new species and products for commercialization. This volume should allow readers at all levels an entry into the exciting world of algal research.

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.