Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.

The study provides a current perspective of the capabilities in genetics and cell biology which have evolved in the last decade and which appear to be of significance for the next decade.

Thirty-four Populus biotechnology chapters, written by 85 authors, are comprised in 5 sections: 1) in vitro culture (micropropagation, somatic embryogenesis, protoplasts, somaclonal variation, and germplasm preservation); 2) transformation and foreign gene expression; 3) molecular biology (molecular/genetic characterization); 4) biotic and abiotic resistance (disease, insect, and pollution); and 5) biotechnological applications (wood properties, flowering, phytoremediation, breeding, commercialization, economics, and bioethics).

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

During the twentieth century, medicine has been radically transformed and powerfully transformative. In 1900, western medicine was important to philanthropy and public health, but it was marginal to the state, the industrial economy and the welfare of most individuals. It is now central to these aspects of life. Our prospects seem increasingly dependent on the progress of bio-medical sciences and genetic technologies which promise to reshape future generations. The editors of Medicine in the Twentieth Century have commissioned over forty authoritative essays, written by historical specialists but intended for general audiences. Some concentrate on the political economy of medicine and health as it changed from period to period and varied between countries, others focus on understandings of the body, and a third set of essays explores transformations in some of the theatres of medicine and the changing experiences of different categories of practitioners and patients.

The book aims to introduce the reader to the emerging field of Evolutionary Systems Biology, which approaches classical systems biology questions within an evolutionary framework. An evolutionary approach might allow understanding the significance of observed diversity, uncover “evolutionary design principles” and extend predictions made in model organisms to others. In addition, evolutionary systems biology can generate new insights into the adaptive landscape by combining molecular systems biology models and evolutionary simulations. This insight can enable the development of more detailed mechanistic evolutionary hypotheses.

Advances in Biological Science Research: A Practical Approach provides discussions on diverse research topics and methods in the biological sciences in a single platform. This book provides the latest technologies, advanced methods, and untapped research areas involved in diverse fields of biological science research such as bioinformatics, proteomics, microbiology, medicinal chemistry, and marine science. Each chapter is written by renowned researchers in their respective fields of biosciences and includes future advancements in life science research. - Discusses various research topics and methods in the biological sciences in a single platform - Comprises the latest updates in advanced research techniques, protocols, and methods in biological sciences - Incorporates the fundamentals, advanced instruments, and applications of life science experiments - Offers troubleshooting for many common problems faced while performing research experiments

PART I Molecular Biology 1. Molecular Biology and Genetic Engineering Definition, History and Scope 2. Chemistry of the Cell: 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids) Sugars (Carbohydrates) 3. Chemistry of the Cell . 2. Macromolecules (Nucleic Acids; Proteins and Polysaccharides) Covalent and Weak Non-covalent Bonds 4. Chemistry of the Gene: Synthesis, Modification and Repair of DNA DNA Replication: General Features 5. Organisation of Genetic Material 1. Packaging of DNA as Nucleosomes in Eukaryotes Techniques Leading to Nucleosome Discovery 6. Organization of Genetic Material 2. Repetitive and Unique DNA Sequences 7. Organization of Genetic Material: 3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic Genes Split Genes or .Interrupted Genes 8. Multigene Families in Eukaryotes 9. Organization of Mitochondrial and Chloroplast Genomes 10. The Genetic Code 11. Protein Synthesis Apparatus Ribosome, Transfer RNA and Aminoacyl-tRNA Synthetases Ribosome 12. Expression of Gene . Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes 13. Expression of Gene: Protein Synthesis: 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes) Polyadenylation of mRNA in Prokaryotes Addition of Cap (m7G) and Tail (Poly A) for mRNA in Eukaryotes 14. Expression of Gene: Protein Synthesis: 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes) Formation of Aminoacyl tRNA 15. Regulation of Gene Expression: 1. Operon Circuits in Bacteria and Other Prokaryotes 16. Regulation of Gene Expression . 2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages 17. Regulation of Gene Expression 3. A Variety of Mechanisms in Eukaryotes (Including Cell Receptors and Cell Signalling) PART II Genetic Engineering 18. Recombinant DNA and Gene Cloning 1. Cloning and Expression Vectors 19. Recombinant DNA and Gene Cloning 2. Chimeric DNA, Molecular Probes and Gene Libraries 20. Polymerase Chain Reaction (PCR) and Gene Amplification 21. Isolation, Sequencing and Synthesis of Genes 22. Proteins: Separation, Purification and Identification 23. Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines 24. Immunotechnology 2. T-Cell Receptors and MHC Restriction 25. Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs) Hybridoma Technology and the Production of Monoclonal Antibodies 26. Transfection Methods and Transgenic Animals 27. Animal and Human Genomics: Molecular Maps and Genome Sequences Molecular Markers 28. Biotechnology in Medicine: l.Vaccines, Diagnostics and Forensics Animal and Human Health Care 29. Biotechnology in Medicine 2. Gene Therapy Human Diseases Targeted for Gene Therapy Vectors and Other Delivery Systems for Gene Therapy 30. Biotechnology in Medicine: 3. Pharmacogenetics / Pharmacogenomics and Personalized Medicine Phannacogenetics and Personalized 31. Plant Cell and Tissue Culture' Production and Uses of Haploids 32. Gene Transfer Methods in Plants 33. Transgenic Plants . Genetically Modified (GM) Crops and Floricultural Plants 34. Plant Genomics: 35. Genetically Engineered Microbes (GEMs) and Microbial Genomics References