Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

The concept of a metabolic profile was introduced in 1971, when gas chromatography demonstrated a range of compounds present in human samples. Now termed metabolomics, the field is still emerging, and chromatography remains an essential tool for determining metabolites in a living system. This is the first book to present the chromatographic techniques used in metabolomics in a fundamental way. Sample preparation and quality control are described in detail, and all forms of chromatography applied to metabolomics are included. The editors present guidelines on selecting the most appropriate methodology, making the book an accessible guide to anyone entering the field. Handling data and applications are also described. This is an essential handbook for any laboratory looking to embark on a metabolomics research programme and includes the fundamentals of chromatography alongside the latest developments in the field.

Metabolism is the sum of the chemical reactions in cells that produce life-sustaining chemical energy and metabolites. In the post-genome era, metabolism has taken on new significance for biological scientists: metabolites are the chemical basis of phenotypes that are final expressions of genomic information. This book covers research on metabolomics, ranging from the development of specialized chemical analytical techniques to the construction of databases and methods for metabolic simulation. The authors have been directly involved in the development of all the subject areas, including capillary electrophoresis, liquid chromatography, mass spectrometry, metabolic databases, and metabolic simulation. Breakthrough achievements and the future of metabolome studies are described, making this book a valuable source for researchers in metabolomics in diverse fields, such as plant, animal, cellular, microbial, pharmaceutical, medical, and genetic sciences.

A new book in the acclaimed Nutrition Society Textbook Series, Nutrition Research Methodologies addresses the rapidly advancing field of nutrition research. It covers the diverse methodologies required for robust nutritional research to ensure thorough understanding of key concepts, both for students at undergraduate and postgraduate levels and for scientists working in nutrition research. Combining theory with practical application, Nutrition Research Methodologies addresses both traditional research methods and new technologies, and focuses on a range of complex topics, including energy compensation, nutrient-gene interactions and metabolic adaptation. It also considers statistical issues as well as application of data to policy development. Provides the reader with the required scientific basics of nutrition research in the context of a systems and health approach Written specifically to meet the needs of individuals involved in nutrition research Combines the viewpoints of world-leading nutrition experts from academia and research with practical applications Accompanied by a companion website with a range of self-assessment material (www.wiley.com/go/lovegrove/nutritionresearch)

Metabolomics is the methodology and theory to study the metabolome, including targeted approaches based on selected/multiple reaction monitoring (SRM/MRM) and untargeted approaches based on nuclear magnetic resonance (NMR) or mass spectrometry (MS). The metabolome contains all metabolites derived from sugars, lipids, proteins, and nucleic acids in a given biological system, tissue, cell, or body fluid in a metabolic network system. Metabolomic variations directly link to molecular mechanisms of a disease, reliable therapeutic targets, and effective biomarkers for prediction, diagnosis, and prognostic assessment of disease. This book presents new advances in the concept and methodology of metabolomics, as well as applications of metabolomics in the research and practice of medical and life sciences.

Metabolomics Perspectives: From Theory to Practical Application is an expertly written volume, which provides a thorough description of the current state-of-the-art in the metabolomics field. The philosophy behind the book is to guide the reader in a step-by-step exploration of metabolomics experiments, ranging from sample preparation to data extraction, analysis and interpretation, and to discuss the main current applications and future perspectives of this emerging science. Armed with critical insights, coupled with a clear writing, the book consists of three main sections. The first one introduces the pivotal theoretical fundamentals and provides a comprehensive overview of the "wet" laboratory workflow, including protocol instructions and a detailed description of experimental methods and analytical techniques. The second section covers a wide range of topics in the context of data analysis, including guidance in exploratory analysis, supervised and unsupervised machine learning approaches and validation and optimization methods. In addition to the several examples reported in the text, the book features an R package, specifically designed to perform all the described algorithms, which is hosted on a companion website (www.metabolomicsperspectives.com) together with several sets of available metabolomic data. Finally, an extensive dissertation describes the latest advances and the major fields of interest for metabolomics applications, highlighting their crucial potentials for future biomedical research. Thus, this book represents a must-read for both experienced researchers, interested in metabolomics, and newcomers to the field. - Provides an in-depth description of the metabolomics experimental workflow and its applications in life science and biomedical research - Features chapter contributions from the greatest international experts in the field - Includes an R package and several sets of metabolomics data, hosted on a companion website

Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees.