Methods in Neurosciences, Volume 4: Electrophysiology and Microinjection is a collection of papers that deals with the electrically excitability feature of many cell types. This volume describes the characteristic features of some nervous tissue to conduct signals along cellular paths or ""wires."" The text presents such paths as a way stimuli are transferred in the nervous system. Section I reviews the recording methodologies such as those used in measuring noninactivating calcium current in smooth muscles cells or the two-suction electrode voltage-clamp recording. Section II deals in detail with voltage clamping and voltammetry; the text also explains the practical steps in using the current pump-assisted voltage clamp. One paper examines the X-ray microprobe analysis of voltage clamped single heart ventricular myocytes, while another paper explains in vivo voltammetry. Section III addresses electrophysiology and purification of specific receptors; one paper presents the results of an electrophysiological study of hormone secretion by single adenohypophyseal cells. Section IV describes special electrodes and equipment, while Section V deals with special preparations needed in culture preparation or in the study of pharmacology of excitatory amino acids on neurons found in the central nervous system. Chapter VI addresses data analysis and reduction such as digital filtering of bioelectric potentials in personal computers. This book will prove valuable for microbiologists, cellular scientists, microchemists, and academicians working in the fields of neuroscience.

Theory and Method in the Neurosciences surveys the nature and structure of theories in contemporary neuroscience, exploring many of its methodological techniques and problems. The essays in this volume from the Pittsburgh -Konstanz series explore basic questions about how to relate theories of neuroscience and cognition, the multilevel character of such theories, and their experimental bases. Philosophers and scientists (and some who are both) examine the topics of explanation and mechanisms, simulation and computation, imaging and animal models that raise questions about the forefront of research in cognitive neuroscience. Their work will stimulate new thinking in anyone interested in the mind or brain and in recent theories of their connections.

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.

Straightforward and practical, this is the first book to provide detailed guidance for using neurobiological methods in the study of human social behavior, personality, and affect. Each chapter clearly introduces the method at hand, provides examples of the method's applications, discusses its strengths and limitations, and reviews concrete experimental design considerations. Written by acknowledged experts, chapters cover neuroimaging techniques, genetic measurement, hormonal methods, lesion studies, startle eyeblink responses, facial electromyography, autonomic nervous system responses, and modeling based on neural networks.

Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemi

An overview of the techniques used in modern neuroscience research with the emphasis on showing how different techniques can optimally be combined in the study of problems that arise at some levels of nervous system organization. This is essentially a working tool for the scientist in the laboratory and clinic, providing detailed step-by-step protocols with tips and recommendations. Most chapters and protocols are organized such that they can be used independently, while cross-references between the chapters, a glossary, a list of suppliers and appendices provide further help.

The past few years have witnessed extraordinary advances in molecular genetic techniques and the accumulation of structural genomics information and resources in both human and model organisms. With the development of new technologies and the availability of resources like the sequence of eukaryotic genomes, problems of a previously unthinkable sco