This thesis presents the major viruses affecting three cultivars in Colombia and how experimental approaches in serology, molecular biology, and bioinformatics can be adapted to create a protocol that could be applied for physalis, passionfruit, ornamental rose, and eventually other crops. Selection of farms was carried out based on size and economic importance. Initial visual plant symptom appraisal led collection of 370 samples from 20 farms. Literature review guided the selection of antibodies of known viruses for initial ELISA testing. Thereafter NGS was carried out to discover further known and novel viruses in the cultivars. Various experiments were carried out on different samples including biotest inoculation, ELISA, RT-PCR, and NGS. Reliable and practical diagnostic tools like these could be developed for the most important viruses leading to a national agricultural certification program that could be established in a common project between German and Colombian universities, the Colombian Agricultural Institute (ICA), the Colombian Corporation of Agricultural Investigation (AGROSAVIA), and the International Center for Tropical Agriculture (CIAT). In dieser Arbeit werden die wichtigsten Viren vorgestellt, die drei Sorten in Kolumbien betreffen, und wie experimentelle Ansätze in den Bereichen Serologie, Molekularbiologie und Bioinformatik angepasst werden können, um ein Protokoll zu erstellen, das für Physalis, Passionsfrucht, Zierrose und eventuell andere Kulturen angewendet werden kann. Die Auswahl der Betriebe erfolgte nach Größe und wirtschaftlicher Bedeutung. Die erste visuelle Beurteilung der Pflanzensymptome führte zur Entnahme von 370 Proben aus 20 Betrieben. Die Literaturrecherche leitete die Auswahl von Antikörpern bekannter Viren für erste ELISA-Tests. Danach wurde NGS durchgeführt, um weitere bekannte und neuartige Viren in den Sorten zu entdecken. Verschiedene Experimente wurden an verschiedenen Proben durchgeführt, einschließlich Biotest-Inokulation, ELISA, RT-PCR und NGS. Solche zuverlässigen und praktischen Diagnosewerkzeuge könnten für die wichtigsten Viren entwickelt werden, die zu einem nationalen Agrarzertifizierungsprogramm führen, das in einem gemeinsamen Projekt zwischen deutschen und kolumbianischen Universitäten, dem Colombian Agricultural Institute (ICA) und der Colombian Corporation of Agricultural Investigation (AGROSAVIA), und das Internationale Zentrum für tropische Landwirtschaft (CIAT) eingerichtet werden könnte.

Strategies to Improve the Quality of Foods, Volume One in the Developments in Food Quality and Safety series explores salt, sugar and fat reduction, while also discussing natural alternatives and nitrate and nitrate salts. Enrichment of foods with prebiotics, probiotics and pos-biotics in food development is also explored. This series is the most up-to-date resource covering trend topics such as Advances in the analysis of toxic compounds and control of food poisoning; Food fraud, traceability and authenticity; Revalorization of agrifood industry; Natural antimicrobial compounds and application to improve the preservation of food; Non-thermal processing technologies in the food industry, and more. Edited by Dr. José Manuel Lorenzo and authored by a team of global experts in the fields of Food Quality and Safety, this series provides comprehensive knowledge to food industry personals and scientists. - Provides latest information regarding the production of food products with modified composition (reformulation) - Brings modern strategies adopted by the food industry to obtain healthier foods without giving up the highest quality standards - Presents salt, sugar, and fat reduction strategies in food products

The expansion of agricultural production in China has been remarkable, but at the expense of the sustainable use of its natural resources. To counter this, as well as to face problems due to rising labour costs and a rapidly ageing rural population, agricultural production must concentrate on a ...

Innovation Strategies for the Food Industry: Tools for Implementation, Second Edition explores how process technologies and innovations are implemented in the food industry, by i.e., detecting problems and providing answers to questions of modern applications. As in all science sectors, Internet and big data have brought a renaissance of changes in the way academics and researchers communicate and collaborate, and in the way that the food industry develops. The new edition covers emerging skills of food technologists and the integration of food science and technology knowledge into the food chain. This handbook is ideal for all relevant actors in the food sector (professors, researchers, students and professionals) as well as for anyone dealing with food science and technology, new products development and food industry. - Includes the latest trend on training requirements for the agro-food industry - Highlights new technical skills and profiles of modern food scientists and technologists for professional development - Presents new case studies to support research activities in the food sector, including product and process innovation - Covers topics on collaboration, entrepreneurship, Big Data and the Internet of Things

This work introduces the concept of reformulation, a relatively new strategy to develop foods with beneficial properties. Food reformulation by definition is the act of re-designing an existing, often popular, processed food product with the primary objective of making it healthier. In recent years the concept of food reformulation has evolved significantly as additional benefits of re-designing food have become apparent. In addition to targeting specific food ingredients that are considered potentially harmful for human health, food reformulation can also be effectively used as a strategy to make foods more nutritious by introducing essential macro- /micro-nutrients or phytochemicals in the diet. Reformulating foods can also improve sustainability by introducing “waste” (and underutilized) ingredients into the food chain. In light of these developments, reformulating existing foods is now considered a realistic and attractive opportunity to provide healthy, nutritious, and sustainable food choices to the consumers and likewise improve public health. Indeed reformulation has now become essential in many cases for redressing the health properties of foods that are popularly consumed and significantly affecting public health. This edited volume covers aspects of food reformulation from various angles, exploring the role of the food industry, academia, and consumers in developing new products. Some of the major themes contributors address include methods of reformulating food products for health, improving the nutritional composition of foods, and challenges to the food industry, including regulation as well as consumer perception of new products. The book presents several case studies to clarify these objectives and illustrate the difficulties encountered in the process of developing a reformulated product. Chapters from experts in the field identify emerging and future trends in food product development, and highlight ways in which these efforts will help with increasing food security, improving nutrition and health, and promoting sustainable production. The editors have designed the book to be useful for both industry professionals and the research community. This interdisciplinary approach incorporates a wide spectrum of food sciences (including composition, engineering, and chemistry) as well as nutrition and public health. Food and nutrition professionals, policy makers, health care and social scientists, and graduate students will also find the information relevant.

The aim of the food processing is to ensure microbiological and chemical safety of foods, adequate nutrient content and bioavailability and acceptability to the consumer with regard to sensory properties and ease of preparation. Processing may have either beneficial or harmful effects on these properties, so each of these factors must be taken into account in the design and preparation of foods. This book offers a unique dealing with the subject and provides not only an update of state-of-the art techniques in many critical areas of food processing and quality assessment, but also the development of value added products from food waste, safety and nanotechnology in the food and agriculture industry and looks into the future by defining current obstacles and future research goals. This book is not intended to serve as an encyclopedic review of the subject. However, the various chapters incorporate both theoretical and practical aspects and may serve as baseline information for future research through which significant development is possible.

Postharvest Management of Fresh Produce: Recent Advances critically addresses the latest issues, challenges, and technological advancements in postharvest management of fresh commodities, especially fruits, nuts, and vegetables. The book covers the intriguing correlation of preharvest treatments, maturity indices and postharvest operations that significantly affect the postharvest quality of fresh produce. Further topics include packaging, logistics and storage technologies, the role of microbial communities, and 'omics' strategies in postharvest disease management. Special attention is given to the latest trends of nanotechnology, internet of things (IoTs), and blockchain technologies in food supply chain management of perishable products. The book is a great resource for young and experienced professionals in academia, industry, and UG/PG students to explore a diversified range of topics in postharvest strategies relevant to food processing, food technologies, agro-processing and quality control. - Thoroughly explores major preharvest losses due to non-availabilities of the latest technologies - Describes the latest trends in the supply chain to minimize preharvest losses - Provides an overview on smart technologies such as nanotechnology, IoTs and blockchain technology