Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades. The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research. The book begins with coverage of super-resolution optical microscopy, which discusses targeted modulation such as STED and SIM or localization methods such as PALM. It then discusses novel development of fluorescent probes, such as organic small-molecule probes, fluorescent proteins, and inorganic labels such as quantum dots. Finally, it describes advanced optical microscopy, such as fluorescence lifetime imaging, fiber optic microscopy, scanning ion conductance microscopy, and the joining of optics and acoustics—photoacoustic microscopy. Following each chapter, a detailed list of references is provided. Problems at the end of each chapter are also included.

How can we obtain tools able to process and exchange information at the molecular scale? In order to do this, it is necessary to activate and detect single molecules under controlled conditions. This book focuses on the generation of biologically-inspired molecular devices. These devices are based on the developments of new photonic tools able to activate and stimulate single molecule machines. Additionally, new light sensitive molecules can be selectively activated by photonic tools. These technological innovations will provide a way to control activation of single light-sensitive molecules, allowing the investigation of molecular computation in a biological environment.

Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

In 1858, Drs. Henry Gray and Henry Vandyke Carter created a book for their surgical colleagues that established an enduring standard among anatomical texts. After more than 150 years of continuous publication, Gray's Anatomy remains the definitive, comprehensive reference on the subject, offering ready access to the information you need to ensure safe, effective practice. This 41st edition has been meticulously revised and updated throughout, reflecting the very latest understanding of clinical anatomy from field leaders around the world. The book's traditional lavish art programme and clear text have been further honed and enhanced, while major advances in imaging techniques and the new insights they bring are fully captured in new state-of-the-art X-ray, CT, MR, and ultrasonic images. - Presents the most detailed and dependable coverage of anatomy available anywhere. - Regional organization collects all relevant material on each body area together in one place, making access to core information easier for clinical readers. - Anatomical information is matched with key clinical information where relevant. - Numerous clinical discussions emphasize considerations that may affect medical care. - Each chapter has been edited by experts in their field, ensuring access to the very latest evidence-based information on that topic. - More than 1,000 completely new photographs, including an extensive electronic collection of the latest X-ray, CT, MR, and histological images. - The downloadable Expert Consult eBook version included with your purchase allows you to search all of the text, figures, references and videos from the book on a variety of devices. - Carefully selected electronic enhancements include additional text, tables, illustrations, labelled imaging and videos – as well as 24 specially invited 'Commentaries' on new and emerging topics related to anatomy.

In the 1960s, Sydney Brenner proposed to use the nematode worm Caenorhabditis elegans to discover the control mechanisms of animal development and to reveal how a small number of neurons generate different behaviours, giving birth to a vibrant community that uses this animal model for their studies. Brenner was aided in his aim by John Sulston, who mapped the C. elegans cell lineages – from a single cell to the multicellular adult – which transformed the field of developmental biology. As a tribute to these two men, this book captures the perspectives of some of the early pioneers of the worm community, from Martin Chalfie, Robert Waterston and Donald Moerman to Catherine Rankin, Antony Stretton and John White. It also includes contributions from subsequent generations of the community, who explore the development and function of the C. elegans nervous system. This book features how this animal has become one of the best models for elucidating the biology of different sensory modalities and their complex behavioural outputs, or how this animal’s survival strategies have contributed to our understanding of ageing and neurodegeneration. Thus, this volume documents the development of the C. elegans neuroscience field, from infancy to maturity. The chapters in this book were originally published as a special issue of the Journal of Neurogenetics.

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

This book will address the advances, applications, research results, and emerging areas of optics, photonics, computational approaches, nano-photonics, bio-photonics, with applications in information systems. The objectives are to bring together novel approaches, analysis, models, and technologies that enhance sensing, measurement, processing, interpretation, and visualization of information. The book will concentrate on new approaches to information systems, including integration of computational algorithms, bio-inspired models, photonics technologies, information security, bio-photonics, and nano-photonics. Applications include bio-photonics, digitally enhanced sensing and imaging systems, multi-dimensional optical imaging and image processing, bio-inspired imaging, 3D visualization, 3D displays, imaging on nano-scale, quantum optics, super resolution imaging, photonics for biological applications, microscopy, information optics, and holographic information systems.

Optical devices in communication and computation have a significant impact on our daily life, although we may not even be aware of their existence, as in case of inter-continent fiber cables that connect people around the world, making it a global village. Novel nanoscale structures have demonstrated a wide range of unique features; therefore have became a hot research topic. Not only that the novel structural materials are used in biomedical therapy, but also the nature inspires the design of innovative optical structures. In this book, we focus on recent developments of theoretical analysis, designs of novel nano-photonic structures and functional materials for optical instrumentation. This book is constituted of 10 chapters contributed by renowned researchers from all over the world who work in the forefront of this field.

This book builds a narrative on the near-field optical and spectroscopic studies with an emphasis on plasmonic- and photonic-assisted nano-optics as a tool for superlensing. Deliberations on near-field studies using confined light in various applications are included along with their commercial implications. Single-molecule detection utilizing efficient surface-enhanced Raman scattering phenomenon in the far-field and plasmonic tip-enhanced Raman scattering studies in the near-field measurements for fast analysis up to trace level is discussed. Features: Covers the broad area of nano-optical spectroscopy from the perspective of putting the concepts and innovations in the field to use Discusses entire spectra of near-field optics and spectroscopy using light Explores gas/chemical sensing using surface plasmon resonance (SPR) in the Kretschmann configuration Includes dielectric nano-photonics and optical confinement Studies phonon behaviour using confined light for the analysis of chemical, biological, and other materials This book is aimed at graduate students and researchers in material science, analytical chemistry, nanotechnology, and electrical engineering.