Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.

Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.

All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Plant cell cultures are used extensively in studies of secondary metabolism, for the biosynthesis of pharmaceuticals, flavors, essences, and pigments. This book highlights recent developments in the in vitro growth of cultured plant cells and in the production of valuable secondary metabolites. Plant Cell Culture Secondary Metabolism details research on many exciting areas including:

Commercial development of cultured-derived food ingredients has attracted interna tional interest. As consumers have become more health conscious in recent years, the de mand for natural food ingredients and disease-preventative phytochemicals has increased tremendously. Plant Cell and Tissue Culture provides an alternative method for controlled production of these products. A wide range of food ingredients has been shown to be pro duced in culture. Much progress has been made in advancing this technology to the point that large-scale production has become possible. This book is developed from the Symposium "Plant Cell and Tissue Culture for Food Ingredient Production" which was held on April 13-17, 1997 at the American Chemical So ciety National Meeting in San Francisco, CA. In this book, international experts in acade mia, government, and industry discuss current advances in the field of plant cell and tissue culture with special emphasis on its application for food ingredient production. Topics re lated to various aspects of plant cell and tissue culture technology are discussed, including overviews of recent advances in plant metabolic pathway studies, process development for improving yields, and bioreactor design and operation for large-scale production. Economic considerations and issues related to the commercial development of culture-derived food in gredients are discussed. Also included are the safety assessment schemes and regulatory frameworks set up by regulatory agencies around the world.

The first volume of this Plant Tissue Culture (PTC) bibliography, published in 1986, covered the literature from 1900 to 1985. This supplement to the PTC bibliography covers the papers and books published in the period 1986-1989, with some references of 1985 and before which were not available when the original volume was compiled. The detailed Introduction to this volume includes a table with a graphic presentation of the trend of research in the field of PTC during this 5-year period. It is interesting to note that during the past five years alone about 6000 papers have been published, which is almost half of the literature published from 1900 to 1985. Another table lists the periodicals and their respective volumes surveyed for this book. Appended is an Index to Plant Names, which facilitates the search of the literature either subject-wise or species/crop-wise.