A balanced treatment of both classical cell biology and modern molecular biology issues. This second edition has been revised to update all scientific content and references. Developed to be a readable story that is accessible, interesting and comprehensible for all introductory students the authors provide a balanced treatment of both classical cell biology and modern molecular biology issues. Students are further presented with historical and experimental approaches to explain the evolution of models and ideas, and to provide actual data for each concept.

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Written for undergraduate cell biology courses, Principles of Cell Biology, Second Edition provides students with the formula for understanding the fundamental concepts of cell biology. This practical text focuses on the underlying principles that illustrate both how cells function as well as how we study them. It identifies 10 specific principles of cell biology and devotes a separate chapter to illustrate each. The result is a shift away from the traditional focus on technical details and towards a more integrative view of cellular activity that is flexible and can be tailored to suit students with a broad range of backgrounds.

The Cell—Prokaryotic and Eukaryotic Cell Organelles: Structure and Function Microscopy and Micrometry Virus World Bacterial Genetics Cellular Reproduction and Death Eukaryotic Chromosomes and Variation DNA—Chemical Nature,Structure and Replication DNA Mutability and its Repair Mechanism Transcription—The Synthesis of RNA Translation—The Synthesis of Protein Regulation of Bacterial Gene Expression Appendix Glossary References Index

This book covers the concept and advances in cell biology with an emphasis on molecular paradigm. It introduces better understanding of molecular concepts and their integral role in structure and function of cell as a basic unit of life and also their integrative role of overall organization of organs. Cell biology is a fascinating branch of biological sciences, providing answers to hitherto unanswered questions. It is the mother science to areas such as molecular biology, molecular genetics, biotechnology, recombinant DNA technology etc. During the last few decades, the science of cell biology has grown at an unprecedented pace with the consequence that voluminous information has accumulated on the subject. Cell and molecular biology is an every dynamic area of life sciences where the core activity of all biological developments are studied in depth. This comprehensive book provides a concise coverage of every topic in cell and molecular biology from the fundamental aspects to the latest developments in a simple and lively manner. The present book titled Cell and Molecular Biology deals with both gross and molecular structure of cell in all its structural and functional manifestations. There are also chapters on genetic engineering and immunology as the understanding of these are very vital for comprehending the expressions of cell machinery.

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.

Developmental biology is at the core of all biology. This text emphasises the principles and key developments in order to provide an approach and style that will appeal to students at all levels.

The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.

The ability to regulate cell volume in the face of osmotic challenge is one of the most fundamental of cellular homeostatic mechanisms. Cellular and Molecular Physiology of Cell Volume Regulation is an integrated collection of articles describing key aspects of cell volume control. The book has been organized around concepts and cellular/molecular processes rather than around mechanisms of volume regulation in specific cell types in order to make it more accessible to a multidisciplinary audience of students, instructors, and researchers.