The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (UK),H. Robert Horvitz (US)and John E. Sulston (UK) "for their discoveries concerning genetic regulation of organ development and programmed cell death." Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death.*Provides the necessary, trusted methods to carry out this research on the latest techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies *Presents key methods on studying tumors and how these cancer cells evade cell death*Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over

One of the major goals of researchers in the field of apoptosis is to identify targets for novel therapies in cancer, AIDS, and Alzheimer's disease. Understanding the molecular mechanisms of the various components of the apoptotic pathways is the first step to reaching this goal. The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK) "for their discoveries concerning genetic regulation of organ development and programmed cell death." Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death. - This volume provides the necessary, trusted methods to carry out this research on these latest therapeutic techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies - Presents key methods on studying tumors and how these cancer cells evade cell death - Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over

Although general morphological features have been used to consistently identify the changes in cell ultrastructure occurring during apoptosis, as distinct from necrosis, important advances have been achieved more recently in the investigation of the cellular and molecular aspects of this process. This book brings together the latest international research on the complex subject of programmed cell death, and covers such areas as the biochemical mechanisms, introduction of DNA fragmentation, enetic regulation, and the importance of apoptosis in the immune system, particularly during T-cell development, and in cancer. The comparison of a number of common signal transduction pathways with those involved in cell growth highlights an important relationship between apoptosis and the control of cell proliferation.

This book introduces readers to the molecules involved in apoptosis and genomal integrity and considers the gain or loss of the functions that lead to cancer.

The molecular mechanisms which determine whether the cells of a multicellular organism will live or commit suicide have become a popular field of research in biology during the last decade. Cell death research in the plant field has also been expanding rapidly in the past 5 years. This special volume of Plant Molecular Biology seeks to bring together examples of a diverse array of experimental approaches in a single volume. From the differentiation of tracheary elements in vascular plants to the more specialized cell death model of the aleurone in cereals, this volume will bring the reader up-to-date with the characterization of different plant model systems that are currently being studied. This endeavor should complement general overviews of plant cell death mechanisms that have been published elsewhere by providing more detailed information on various aspects of this field to interested graduate students and more senior biologists alike.

Under the name of programmed cell death (PCD) are included diverse molecular mechanisms of cell suicide which play an essential role in the development of multicellular organisms. The best known PCD mechanism in multicellular organisms is called apoptosis. However, recent studies indicate that PCD is also present in protozoa and unicellular eukaryotes. The eleven chapters of this book give the reader a comprehensive update of the progress in the understanding of the mechanisms of PCD in protozoa. The chapters have been written by experts in this field of research and are arranged following an evolutionary point of view.

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Thoroughly revised and updated, this comprehensive and general gynecological textbook provides guidance for the management of specific gynecological conditions.