Introduces the basic principles and techniques of recombinant DNA. The book begins with an introduction to the different tools used for gene cloning. The final chapters cover the application of Recombinant Technology to current research and provide an inside look at the human genome project, ribozyme technology, antisense technology, DNA sequencing, and protein engineering.

Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.

Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

Recombinant DNA technology is a technique which changes the phenotype of an organism (host) when a genetically altered vector is introduced and integrated into the genome of the organism. So, basically the process involves the introduction of a foreign piece of DNA structure into the genome which contains our gene of interest. This gene which is introduced is the recombinant gene and the technique is called the recombinant DNA technology. Inserting a desired gene into the genome of the host is not as easy as it sounds. It involves the selection of the desired gene for administration into the host followed by a selection of the perfect vector with which the gene has to be integrated and recombinant DNA formed. This recombinant DNA then has to be introduced into the host. And at last it has to be maintained in the host and carried forward to the offsprings. In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmic, Lambda phages). A vector containing foreign DNA is termed recombinant DNA. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Of these, the most commonly used vectors are plasmids. Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. Recombinant DNA Technology is focuses on the current state of knowledge on recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

I am very glad to present this book of Basic Concept of Recombinant DNA Technology, written according to revised syllabus of B.Sc, M.Sc(Biotechnology, Microbiology), B.Pharm, M.Pharm, M.Sc Agriculture and Veterinary in all Indian Universities. This book is also useful for the medical students. I extend my good wishes to the students and teachers of Biotechnology and Microbiology, sincerely hope that Basic Concept of Recombinant DNA Technology, will receive a warm welcome from them. I welcome comments by readers of Basic Concept of Recombinant DNA Technology, for way to improve the book and to increase its value. Such suggestions will be seriously considered in the preparation of subsequent editions. I am very grateful to Dr. Tanusri Mandal, Associate Professor and Head, Department of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, India for useful suggestions and help made by her time to time. Finally, I would like to thanks my wife Arpita Pattanayak(De), and my sweet daughter Anindita De for continuous encouragement for completion of this book.

For the new nanotechnology entrepreneur, starting up a venture requires concise navigation through a sea of developmental red tape. This predicament is true of any startup, nano or not, but is particularly exacerbated by the fact that nanotechnology is a new and potentially disruptive technology. A unique exposition on starting and running a nano-business, this indispensible reference: Includes samples of important corporate and operational documents Explores the intricate relationship between new technology development and the creation of new businesses Provides tips on managing people of diverse educational backgrounds Incorporates information on patents, business ethics, record keeping, and marketing Nanotechnology: The Business presents an in-depth discussion of available corporate structures, delineating the advantages and disadvantages of each. It also describes an array of other issues the nano entrepreneur will encounter, from business plans and financing to budgeting, facilities procurement, and staffing. With a scope like no other book of its size, this handy guide equips nano entrepreneurs with the expertise needed to sail smoothly through startup and ensure successful operations after initial incorporation.

Pharmaceutical Biotechnology offers students taking Pharmacy and related Medical and Pharmaceutical courses a comprehensive introduction to the fast-moving area of biopharmaceuticals. With a particular focus on the subject taken from a pharmaceutical perspective, initial chapters offer a broad introduction to protein science and recombinant DNA technology- key areas that underpin the whole subject. Subsequent chapters focus upon the development, production and analysis of these substances. Finally the book moves on to explore the science, biotechnology and medical applications of specific biotech products categories. These include not only protein-based substances but also nucleic acid and cell-based products. introduces essential principles underlining modern biotechnology- recombinant DNA technology and protein science an invaluable introduction to this fast-moving subject aimed specifically at pharmacy and medical students includes specific ‘product category chapters’ focusing on the pharmaceutical, medical and therapeutic properties of numerous biopharmaceutical products. entire chapter devoted to the principles of genetic engineering and how these drugs are developed. includes numerous relevant case studies to enhance student understanding no prior knowledge of protein structure is assumed

Recombinant DNA and Biotechnology is intended to intrigue a global showing group of onlookers and will empower all educators to instruct a sensible measure of atomic science and hereditary designing to understudies. It is essentially the Insertion of a specific fragment of foreign DNA into a Cell, through a suitable vector, in such a way that inserted DNA replicates independently and transferred to Progenies as a result of Cell Division. The Transformed Cells containing DNA after their characterization and confirmation can be used commercially for the production of useful compounds such as Insulin, Interferon, Growth Hormones, Etc. Recombinant DNA and Biotechnology: A Guide for all will empower to learn course on the fundamental standards, basic lab exercises, and significant social issues and concerns orderly to today's sub-atomic science insurgency.