Visible and near-infrared, known as VIS-NIR spectroscopy has emerged as a highly promising alternative to conventional laborious wet chemistry methods for qualitative and quantitative analysis of samples. VIS-NIR spectroscopy has successfully been employed in the analysis of a wide variety of applications from food quality analysis to environmental monitoring, medical diagnosis and even in arts and archaeology. This book presents the state-of-the-art method for profiling cancerous cells using the VIS-NIR spectra between 400 nm and 1100 nm. What makes our book truly exceptional? The cell culture techniques, spectroscopic experimental setup and statistical methods are presented in detail, conveniently allowing readers to replicate the study. In this book, the study was specifically conducted on mouse skin fibroblast (L929), human cervix adenocarcinoma (HeLa) and human prostate carcinoma (DU145) cell lines. These cell selections are commonly used in cytotoxicity, anti-cancer, antiproliferative and diagnosis research. Whether you're a student specializing in cell studies or a researcher immersed in optical-based instrumentation and measurement techniques, this book is your essential companion for success.