Volume 27 provides a comprehensive review of current knowledge of lysosome function in mammalian cells. The book's unique contribution is its series of chapters that offers unparalleled treatment of the metabolic activities of lysosomes.

Lysosomes are membrane-surrounded organelles which are present in all animal cells. The importance of this organelle is underlined by an increasing number of human diseases, which are associated with an impaired function of the lysosomal compartment. This book summarizes the current state-of-the art knowledge about this unique organelle. It addresses the biogenesis of this compartment, the transport of lysosomal proteins, the role of the lysosomal membrane in lysosomal stability and transport, the function of lysosomal proteases and hydrolases, lysosomal storage disorders, and new concepts on how to treat these diseases. In addition to these classical topics, new insights into lysosomal functions are covered by chapters dealing with specialized lysosomes involved in bone resorption and plasma membrane repair, the lysosomal transciptome, and proteome and the emerging role of lysosomes in special forms of autophagy. This book will provide readers with a comprehensive overview into how this fascinating organelle works and how research in the field is developing.

The field of lysosomal transport has grown exponentially in the past decade. Research in this previously unknown function of lysosomes has resulted in understanding the metabolic defect in three inborn errors in metabolism: nephropathic cystinosis, cobalamin F-deficient methylmalonic aciduria, and Salla disease. Seventeen transport systems mediating the exodus from lysosomes of amino acids, sugars, nucleosides, phosphate, calcium, cobalamin, and sulfate have been described. Pathophysiology of Lysosomal Transport presents the current status in this field as described by the authors who made the original discoveries. Each chapter examines the pathological consequences resulting from a defect in a particular system. The book also examines the transfer of macromolecules into the lysosomes, describes the analogy between mammalian lysosomes and vacuoles of plants and fungi, and reviews non-mediated transport. A comprehensive chapter on the methodology required to perform lysosomal studies will benefit researchers undertaking investigations in this area.

The lysosome system; Occurrence of lysosomes; Physiological functions of lysosomes: role of lusosomes in intracellular digestion; Physiological functions of lysosomes: role of lysosomes in secretion and extracellular digestion; role of lysosomes and acid hydrolases in developmental processes; Lysosomes in disease and injury.

Pathobiology of Cell Membranes, Volume I, represents the first in a multivolume treatise designed for pathologists and cell biologists interested in the role of membrane alterations in disease processes. The treatise will emphasize concepts related to membrane alterations in cell injury as well as methods for elucidating such changes. The book begins with a discussion of the general conceptual aspects of cell injury and some details about the role of membrane alterations in the pathogenesis of both lethal and sublethal cellular reactions. This is followed by separate chapters on applications of circular dichroism to the study of membranes; a cell injury mechanism involving membrane components at the molecular level; and fundamental aspects of cell volume regulation and the modification of this regulation by primary or secondary damage of the cell membrane. Subsequent chapters deal with comparative roles of lysosomal and other membrane damage in cell injury; the role of membrane movements and various types of lysosome formation; toxic effects on mitochondrial membranes; and the concept of a biological membrane in health and disease at the tissue level.

The knowledge of lysosomal biology and the consequences of its dysfunction have increased dramatically in the past 60 years. This book describes the nature of the lysosomal dysfunction and diseases as well as potential future treatments and therapies. Disease specific chapters provide thorough reviews of the clinical features of lysosomal storage disorders, their molecular basis and the commercial or experimental therapeutic approaches sought in this area. This is an invaluable resource for researchers in biochemical and molecular genetics, enzyme therapy, and gene transfer.

Ultrastructural Pathology of the Cell and Matrix: Third Edition Volume 2 presents a comprehensive examination of the intracellular lesion. It discusses the analysis of pathological tissues using electron microscope. It addresses the experimental procedures made on the cellular level. Some of the topics covered in the book are the structure, distribution, and variations of rod-shaped microtubulated bodies; morphology of intracytoplasmic filaments; melanosome-producing and melanosome-containing cells in tumours; myofilaments in striated muscle; and pathological variations in size, shape, and numbers of microbodies. The intracytoplasmic and intranuclear annulate lamellae are fully covered. An in-depth account of the classification, history, and nomenclature of lysosomes are provided. The morphology and normal variations of melanosomes and anchoring fibrils are completely presented. A chapter is devoted to the endocytotic structures and cell processes. Another section focuses on the classification and nomenclature of fibrous components. The book can provide useful information to cytologists, pathologists, students, and researchers.